16th Regiment or 16th Infantry Regiment may refer to:
- 16th Air Defence Regiment, Royal Australian Artillery
 - 16th Alpini Regiment, a short lived light Infantry training regiment of the Italian Army, specializing in Mountain Combat
 - 16th Punjab Regiment (Pakistan)
 - 16th Infantry Regiment (South Korea)
 - 16th Regiment Royal Artillery, a regiment of the Royal Artillery in the British Army
 - 16th The Queen's Lancers, a cavalry regiment in the British Army, first raised in 1759
 - 16th Infantry Regiment (United States), a regiment in the United States Army
 - Bedfordshire and Hertfordshire Regiment, British Army regiment formerly designated the 16th Regiment of Foot
 
United States
American Revolutionary War regiments
- 16th Massachusetts Regiment, a unit of the American Massachusetts Line
 
American Civil War regiments
- 16th Illinois Volunteer Infantry Regiment, nicknamed "The Twins", an infantry regiment that served in the Union Army during the American Civil War
 - 16th Regiment Illinois Volunteer Cavalry, a cavalry regiment that served in the Union Army during the American Civil War
 - 16th Maine Volunteer Infantry Regiment, an infantry regiment that served in the Union Army during the American Civil War
 - 16th Michigan Volunteer Infantry Regiment, an infantry regiment that served in the Union Army during the American Civil War
 - 16th West Virginia Volunteer Infantry Regiment, an infantry regiment
 - 16th Wisconsin Volunteer Infantry Regiment, an infantry regiment that served in the Union Army during the American Civil War
 
See also
- 16th Army (disambiguation)
 - 16th Wing (disambiguation)
 - 16th Group (disambiguation)
 - 16th Division (disambiguation)
 - 16th Brigade (disambiguation)
 - 16th Squadron (disambiguation)
 
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