John Gwynne, Gwynn or Gwyn may refer to:
Politicians
- John W. Gwynne (1889–1972), Republican U.S. Representative from Iowa
 - John Gwynne (MP for Bath) (fl. 1563), English Member of Parliament (MP)
 - John Gwynne (died 1574), MP for Caernarvonshire (UK Parliament constituency)
 - John Gwyn (fl. 1553 – c. 1567), MP for Cardigan (UK Parliament constituency)
 - John Gwynne (fl. 1572), MP for Wareham (UK Parliament constituency)
 
People in sport
- John Tudor Gwynn ("Jack", 1881–1956), Irish cricketer and reporter in British India
 - John David Gwynn (1907–1998), Irish cricketer and civil engineer
 - John Gwynne (commentator) (1945–2022), British sport reporter and commentator
 
Others
- John Gwynne (captain) (fl. 1660), Welsh soldier
 - John Gwynn (architect) (1713–1786), British architect and civil engineer
 - John Gwyn (philanthropist) (1755–1829), Derry linen merchant and benefactor
 - John Wellington Gwynne (1814–1902), Canadian lawyer and Supreme Court Judge
 - John Gwynn (professor) (1827–1917), Irish Syriacist and Anglican dean
 - John Gwynne (author), British writer of fantasy literature
 
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