John Mackie may refer to:
- John C. Mackie (1920–2008), U.S. Representative from Michigan
 - John Duncan Mackie (1887–1978), Scottish historian
 - J. L. Mackie (1917–1981), Australian-born philosopher, best known for his views on meta-ethics
 - John Mackie, Baron John-Mackie (1909–1994), British Labour Member of Parliament 1959–1974
 - John Mackie (Scottish Unionist politician) (1898–1958), Scottish Unionist Member of Parliament for Galloway 1931–1958
 - John F. Mackie (1835–1910), first United States Marine to receive the Medal of Honor
 - John Milton Mackie (1813–1894), American writer
 - John Mackie (Kirkcudbright MP) (died 1858), MP for Kirkcudbright Stewartry
 - John Mackie (born 1961), bassist for Scottish post-punk band Scars
 
Association football
- Alec Mackie (Irish footballer) (John Alexander Mackie, 1903–1984), Irish professional footballer who played for Arsenal, Portsmouth and Northampton Town
 - John Mackie (footballer, born 1910) (1910–1980), Scottish professional footballer who played for Hull City, Bradford City and Chesterfield
 - John Mackie (footballer, born 1976), English professional footballer who played for Reading and Leyton Orient
 
See also
- Jack McKay (disambiguation)
 - John MacKay (disambiguation)
 - John Mackey (disambiguation)
 - John McKay (disambiguation)
 
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