The following highways are numbered 54:
Canada
Finland
India
Italy
Japan
Korea, South
- National Route 54
Mexico
New Zealand
Philippines
Turkey
United Kingdom
United States
 U.S. Route 54 U.S. Route 54
 Alabama State Route 54 Alabama State Route 54
 Arkansas Highway 54 Arkansas Highway 54
 California State Route 54 California State Route 54
 Delaware Route 54 Delaware Route 54
 Florida State Road 54 Florida State Road 54
 Georgia State Route 54 Georgia State Route 54.svg.png.webp) Georgia State Route 54B (former) Georgia State Route 54B (former)
 
 Idaho State Highway 54 Idaho State Highway 54
 Illinois Route 54 Illinois Route 54
 Indiana State Road 54 Indiana State Road 54
 Kentucky Route 54 Kentucky Route 54
 Louisiana Highway 54 Louisiana Highway 54
 Maryland Route 54 Maryland Route 54
 M-54 (Michigan highway) M-54 (Michigan highway)
 Minnesota State Highway 54 Minnesota State Highway 54
- Missouri Route 54 (1922) (former)
 Nebraska Highway 54 (former) Nebraska Highway 54 (former)
 Nevada State Route 54 (former) Nevada State Route 54 (former)
 New Jersey Route 54 New Jersey Route 54
 New York State Route 54 New York State Route 54 County Route 54 (Cayuga County, New York) County Route 54 (Cayuga County, New York)
 County Route 54 (Chautauqua County, New York) County Route 54 (Chautauqua County, New York)
 County Route 54 (Chemung County, New York) County Route 54 (Chemung County, New York)
 County Route 54 (Dutchess County, New York) County Route 54 (Dutchess County, New York)
 County Route 54 (Franklin County, New York) County Route 54 (Franklin County, New York)
 County Route 54 (Jefferson County, New York) County Route 54 (Jefferson County, New York)
 County Route 54 (Lewis County, New York) County Route 54 (Lewis County, New York)
 County Route 54 (Orange County, New York) County Route 54 (Orange County, New York)
 County Route 54 (Oswego County, New York) County Route 54 (Oswego County, New York)
 County Route 54 (Otsego County, New York) County Route 54 (Otsego County, New York)
 County Route 54 (Putnam County, New York) County Route 54 (Putnam County, New York)
 County Route 54 (Rensselaer County, New York) County Route 54 (Rensselaer County, New York)
 County Route 54 (Saratoga County, New York) County Route 54 (Saratoga County, New York)
 County Route 54 (Schoharie County, New York) County Route 54 (Schoharie County, New York)
 County Route 54 (St. Lawrence County, New York) County Route 54 (St. Lawrence County, New York)
 County Route 54 (Suffolk County, New York) County Route 54 (Suffolk County, New York)
 County Route 54 (Tioga County, New York) County Route 54 (Tioga County, New York)
 County Route 54 (Ulster County, New York) County Route 54 (Ulster County, New York)
 County Route 54 (Washington County, New York) County Route 54 (Washington County, New York)
 County Route 54 (Westchester County, New York) County Route 54 (Westchester County, New York)
 County Route 54 (Wyoming County, New York) County Route 54 (Wyoming County, New York)
 
 North Carolina Highway 54 North Carolina Highway 54
.svg.png.webp) North Dakota Highway 54 North Dakota Highway 54
 Ohio State Route 54 Ohio State Route 54
 Oklahoma State Highway 54 Oklahoma State Highway 54
 Pennsylvania Route 54 Pennsylvania Route 54
.svg.png.webp) South Dakota Highway 54 (former) South Dakota Highway 54 (former)
  Tennessee State Route 54 Tennessee State Route 54
 Texas State Highway 54 Texas State Highway 54
 Utah State Route 54 Utah State Route 54 Utah State Route 54 (1931-1969) (former) Utah State Route 54 (1931-1969) (former)
 
 Virginia State Route 54 Virginia State Route 54
 West Virginia Route 54 West Virginia Route 54
 Wisconsin Highway 54 Wisconsin Highway 54
- Territories
 Puerto Rico Highway 54 Puerto Rico Highway 54 Puerto Rico Highway 54R Puerto Rico Highway 54R
 
See also
| Preceded by 53 | Lists of highways 54 | Succeeded by 55 | 
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